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Protocol for Staining SH-SY5Y Cells with C3:0-BODIPY-GM1


N-Propanoyl-BODIPY-monosialoganglioside GM1 (NH4+ salt) - Catalog number 2060

Staining SH-SY5Y Cells with C3-BODIPY-GM1

1. Dissolve C3:0-BODIPY-GM1 (100 μg) in 64 μl DMSO:methanol:water (2:1:0.2) and aliquot. Store aliquots at -20°C until use.
2. Plate cells at density of 10,000/well in a 96-well plate in media with 10% FBS and allow to attach overnight.
3. The following day, remove media and replace with 2.5-10 μM C3:0-BODIPY-GM1 in phenol red-free media. Return cells to incubator for 30 minutes.
4. Remove media and replace with fresh phenol red-free media. Image using a fluorescence microscope with a FITC/GFP laser.

Note that complexing fluorescent lipids with bovine serum albumin (BSA) facilitates cell labeling by eliminating the need for organic solvents to dissolve the lipophilic probe. A BSA-complexed probe can be directly dissolved in water. However, gangliosides are unique lipids that are soluble in aqueous systems.

The above image is SH-SY5Y neuroblastoma cells stained with 10μl C3:0-BODIPY-GM1.

This product is to be used for research only. It is not intended for drug or diagnostic use, human consumption, or to be used in food or food additives. Matreya assumes no liability for any use of this product by the end user. We believe the information, offered in good faith, is accurate.
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