Lissamine-rhodamine B-dodecanoyl-galactosylceramide

Lissamine-rhodamine B-dodecanoyl-galactosylceramide is a fluorescent labeled glycosphingolipid containing a galactose attached to a ceramide and labeled with a fluorescent lissamine-rhodamine B marker. This fluorescent standard from Matreya is excellent for use in the identification and isolation of cerebrosides in the Krabbe’s disease and other studies.3 Lissamine-rhodamine B dyes have an excitation/emission maxima ~560/580 nm. The fluorescent marker is attached via a 12-carbon linker reducing the interaction of the fluorophore with the sphingolipid. Galactosylcerebrosides are found primarily in neuronal tissues and are a major component of the central nervous system. They are the largest single component of the myelin sheath of nerves and seem to act, along with other molecules, to form part of the structural support of the myelin sheath.1 Cerebrosides are involved in a very wide range of biological activities such as cell agglutination, intracellular communication, cellular development, and antitumor/cytotoxic effects.2 Galactocerebroside can be metabolized into sulfatide which is also abundant in the nervous system and myelin sheath. Due to the relatively high melting point of cerebrosides (much greater than physiological body temperature) they have a paracrystalline structure. Krabbe’s disease (globoid cell leukodystrophy) is characterized by a deficiency in the enzyme galactocerebrosidase, which is responsible for degrading galactocerebroside. This leads to an accumulation of cerebroside and psychosine which can result in demyelination of nerves and loss of axonal conductivity.