Biotinylated Ceramides

Biotinylated sphingolipids are ideal for use in sphingolipid research because they take advantage of the strong and specific interaction of biotin with streptavidin and avidin. These sphingolipid analogs contain a biotin label attached to the amine of the sphingosine moiety via a hexanoic acid linker which maintains the sphingolipid’s natural properties. The biotin label allows for easy attachment of the sphingolipid to streptavidin and avidin proteins making them extremely useful for binding to substrates and for toxin detection.^1,2

References:

1. A. Mukhopadhyay et al. Direct interaction between the inhibitor 2 and ceramide via sphingolipid-protein binding is involved in the regulation of protein phosphatase 2A activity and signaling. FASEB, Vol. 23:3 pp. 751-763, 2009
2. A. Pukin et al. Chemoenzymatic synthesis of biotin-appended analogues of gangliosides GM2, GM1, GD1a and GalNAc-GD1a for solid-phase applications and improved ELISA tests. Org. Biomol. Chem., Vol. 9:16 pp. 5809-5815, 2011